AnaEE France has brought together in an integrated network a unique collection of experimental platforms in controlled, semi-natural or natural environments, analysis platforms and shared instruments as well as modeling platforms and Database Information Systems, all devoted to the biology of continental ecosystems. The infrastructure is built around three main service offerings that provide access to a range of experimental facilities: the Très Grande Infrastructure de Recherche des Écotrons - Écotrons Very Large Research Infrastructure, four experimental platforms in semi-natural environments, and some 20 natural sites in metropolitan France and French Guiana where long-term experiments are conducted in forests, pastures, lakes and crops. In the field of environmental microbiology, AnaEE France is also investing in the shared instruments and analytical resources required for a detailed characterization of the environment and microorganisms. In a single integrated network it provides all the tools required to study, understand and model biological systems and conduct innovative biological research on gene - environment interactions, biodiversity and the functioning of ecosystems.

The site of Montiers, localized at the boundaries between Meuse and Haute-Marne departments, North-East of France, has a large surface area (143 ha). It comprises two soil successions (toposequence) and the climate, stand conditions (age, species, forest management) are equivalent on all the surface of the site. The facility comprises three biogeochemical stations of 10 000 m2 each and one flux tower above forest canopy (45 m-high) settled along a soil succession representative of soils of the region. The three stations include four substations of which three are strongly equipped and one is free for future experimentations. Each equipped substation comprises lysimeters at different soil depths (litter, -10 cm, -30 cm, -60 cm and -90 cm; 3 replicates in general), tensio-lysimeters (-10 cm, -30 cm, -60 cm, -90 cm and -120 cm; 3 replicates in general) and temperature and moisture probes at different soil depths (-10 cm, -30 cm, -60 cm and -90 cm; 4 replicates), litterbags (6 replicates), stemflows (6 replicates), gutters (4 replicates). These stations allow to follow-up on the long term the flows of water, and major (Ca, Mg, K, Na, P, Fe, Mn, Si, Al, S, C, N) and trace (Cl, Se, B, I, Cs) elements between the different compartments (soil, tree, atmosphere) of a beech forest. Each station is settled on a different soil type, i.e., alocrisol, calci-brunisol, rendosol thus allowing to assess the impact of the soil type on biogeochemical cycles and on tree growth. The flux tower is equipped with a Eddy Covariance system (CO2, H2O, and sensible heat) and a complete set of sensors recording the aerial and edaphic meterological conditions, the phenology and the canopy status. The data from flux tower are available on demand. The forest mainly consists in a beech timber of about 50 years: dominant species and forestry in the region. The effect of the soil on the biogeochemical and biological functioning of this beech forest is dealt with a very integrated approach (ecophysiology, microbiology, soil science and biogeochemistry). In addition, the flux tower permits to measure, at various levels of the canopy and above, meteorological parameters (temperature, radiation, and precipitation), the gaseous exchanges and the particular deposits.

Data obtained from the INRA CARRTEL laboratory in Thonon-les-Bains (SOERE-OLA: Observatory and Experimentation Systems for Environmental Research - LAkes Observatory) in collaboration with CISALB (Inter-Syndical Committee for Sanitation Lake Bourget). These data cover different topics: The variables of the physico-chemistry theme are: nitrogen, calcium, carbon, chlorides, conductivity, magnesium, dissolved oxygen, pH, phosphorus, potassium, reactive silica, sodium, sulphate, temperature, complete alkalimetric titre. Data taken at various depths throughout the lake's water column. The water column is made at the deepest point of the lake (140 m). The samples are taken every 15 days. The period concerns a follow-up from 1999 to the present. The variables of the chlorophyll theme are: chlorophyll a strickland-parsons, chlorophyll c, carotenoids, chlorophyll a scor-unesco, pheopigments. Data taken at various depths throughout the lake's water column. The samples are taken one to three times a month. The period concerns a follow-up from 2006 to the present. The variables of the topic sampling conditions are: air temperature, cloudiness, sunshine, weather, wind direction, wind speed, atmospheric pressure, water aspect, surface condition, transparency, color of water. Studies are carried out approximately once or twice a month. The period concerns a follow-up from 2010 to the present. The variables of the phytoplankton theme are: determiner name, sedimented volume, counting surface, determined taxon, number of counted fields, number of objects counted, number of objects per ml, number of cells per ml, biovolume of species In the sample. Data taken from an integrated depth of 0 to 10m or 0 to 20m depending on the year. Samples are taken once a month. The period concerns a follow-up from 1995 to the present. The variables of the primary production theme are: duration of incubation, primary production by actual incubation duration, primary production per hour, primary production by median third. Data taken at various depths on the lake's water column from 0 to 30m. The samples are taken once or twice a month. The period concerns a follow-up from 2011 to the present. The variables of the probe theme are: depth, temperature, chlorophyll a, pH conductivity, oxygen, oxygen saturation rate, turbidity, transparency, by w, by a, pah, pc. Data obtained at various depths (every 10 cm approximately) over the whole water column of the lake (0 to 140m). Measurements are performed once or twice a month. The period concerns a follow-up from 2003 to the present. The variables of the zooplankton theme are: name of the determiner, sedimented biovolume, name of the taxon determined, stage of development, number of individuals. Data taken from an integrated depth of 0 to 50m. The samples are taken one to three times a month. The period concerns a follow-up from 1996 to the present (absence of data between 1997 and 2003 inclusive).

With this innovative platform we will be able to bring the laboratory to the field (rather than report samples to the laboratory with all that that implies as disruption and transformation between the in situ sampling and laboratory analysis). It is composed of a number of complimentary and innovative kits (soil,vegetation, micro climate, microbiology..). Each kit contains mobile state of the art equipement able to fullfill a great number of analytic and monitoring tasks.. It will aim: (i) to characterize environmentally terrestrial ecosystems (especially sites non-instrumented but with great potential to generalize the results obtained on the three nodes of the project (eg experimental devices GIS Data Cooperative - or provenance trials - eg Plantacomp) - the platform will be a major-waiting for inter-study ecosystems (forests, grasslands, crops); (ii) allow campaigns targeted measures at highly instrumented monitoring sites or at other representative sites(instantaneous flow capture special events, unstable compounds) (iii) serve as a support for the development of measures in situ and non-destructive analytic techniques and methods. (soil, vegetation….) Overall, this platform is a facility open acces, usuable by the entire community of environmental sciencists.

Data from the INRA CARRTEL laboratory in Thonon-les-Bains (SOERE-OLA: Observation and Experimental Systems for Environmental Research - LAkes Observatory) in collaboration with ASTERS (Conservatory of natural areas, preservation of the natural heritage of Haute-Savoie, nature reserves, fauna and flora species). The sentinel lakes are the lakes of: Anterne, Brévent, Bressse inf, Bresse sup, Horn, Cornu, Cos, Jovet, Lauzanier, Malrif, Muzelle, Pave, Pétarel, Pisses, Plan-Vanniey, Porménaz, Rabuons. These data cover different topics: The variables of the physico-chemistry theme are: nitrogen, calcium, carbon, chlorides, conductivity, magnesium, dissolved oxygen, pH, phosphorus, potassium, reactive silica, sodium, sulphate, temperature, complete alkalimetric titre. Data taken at various depths over the whole water column of the lakes concerned (Anterne, Brévent, Cornu, Jovet, Porménaz). The water column is made at the deepest point of each lake. The samples are taken every 15 days. The period concerns a follow-up from 1992 to the present. The variables of the sampling conditions theme (obtained on all the sentinel lakes) are: air temperature, cloudiness, sunshine, weather, wind direction, wind speed, atmospheric pressure, water aspect, Surface, transparency, color of water. Studies are carried out approximately once or twice a month. The period concerns a follow-up since 2013 (or 2014 according to the lakes) until today. The variables of the phytoplankton theme are: determiner name, sedimented volume, counting surface, determined taxon, number of counted fields, number of objects counted, number of objects per ml, number of cells per ml, biovolume of species In the sample. Data taken from an integrated depth of 0 to 5m, 0 to 17m or 0 to 25m depending on the lakes (Anterne, Brévent, Corne, Cornu, Cos, Jovet, Porménaz, Rabuons). Samples are taken once a month. The period concerns a follow-up since 1998 (or 2015 according to the lakes) until today. The variables of the zooplankton theme are: name of the determiner, sedimented biovolume, name of the taxon determined, stage of development, number of individuals. Data taken from an integrated depth of 0 to 24 m for Lake Corne, 0 to 37 m for Cos Lake and 0 to 44 m for Lake Rabuons. The samples are taken one to three times a month. The period concerns a follow-up from 2015 to the present.

This SOERE aims at understanding and modelling the dynamics and ecological functioning of lake ecosystems affected by local and global forcing factors. Four peri -alpine lakes (Lakes Geneva , Annecy, Bourget, Aiguebelette) and their watersheds are monitored. These systems represent important natural resources and meet key societal demands (drinking water, fishing, tourism, biodiversity ... ) . The services provided by the Soere relates to both long-term in situ observation and experimentation. The observatory task is based on logistic, technical and analytical supports for (i) collecting environmental data (physico- chemical and biological), (ii) creating and managing database (ii) organizing the storage of reference biological samples. The accession to the data is possible according to the 'Terms & Conditions of Use' of the Soere Database. The SOERE OLA can provide logistic supports for sampling, in situ measurements and experimentation on Lake Geneva. This service is submitted to validation according to the " Logistic Support Leman" service charter. Other lake sites have joined the SOERE more recently, especially high lakes (sentinel lakes) and a model lake (meromictic), Lake Pavin. The SOERE OLA has Allenvi label.

The Estrées-Mons platform is dedicated to arable crops. It evaluates the effect of agricultural practices on C and N cycles in the soil-plant system and their interaction. Nitrate leaching, SOM evolution and GHG emissions (CO2, N2O) are monitored according to level of N intensification, crop residues export, soil tillage and legume frequency. The key issue is to understand how the wide variation in C and N inputs affects C and N cycles in more or less intensified systems.

Each site has three common treatments: a control (C), a compacted treatment (T), a compacted and decompacted treatment (D), and more specific ones, locally decompacted soil (P) at Azerailles 54, and, limed (A) and limed and compacted (CA) at Clermont en Argonne 55. Each treatment plot of 0.25ha is replicated three times (3 blocks). Azerailles site was installed in spring 2007 and Clermont in spring 2008. Since that time monitoring occured for: -mesoclimate: rainfall, air temperature, relative humidity, bulk deposition (continuous recording) - soil climate: soil moisture (TDR Trase system (5 replicates at 15 and 60 cm depth) in one replicate of C and T (recorded each four hours) - water table: 2 piezometers in each replicate of treatments (Divers hourly recorded); monthly sampling using specific devices for Redox problems - weakly fixed soil water: cup lysimeters inserted at 15 and 60 cm depth (4 replicates in one block for C and T treatments) + teatments A and CA at Clermont en Argonne. Automate for constant vacuum at 700hP. Monthly collection for chemical analysis - soil gas: specific captors installed at 5, 10, 20, 35, 50 and 70 cm depth in each C and T treatments of the 3 blocks in the 2 sites. Monthly collection for chemical analysis (N, CO2, CH4, N2O, O2) -soil solid phase: soil morphology, soils physics, chemistry and biology (pro parte) were initialy described. Soil physical parameters (strengh, bulk density, hydraulic conductivity, swelling/shrinkage, porosity...), chemistry (C, N, exchangeable cations, P) and biology (earthworms) are perediodicaly investigated (each year for physics; 2/3 years for chemistry and biology). vegetation: both understorey and oak plantation were annually observed soil biology: earthworms are periodically observed

Capsis is a simulation platform for forestry growth and dynamics models. It is a tool for forest scientists, forest managers and education and has been developed in the AMAP laboratory since 1999. Scientists who designed a forestry model may build a simulator in Capsis with the help of a developer according to the Capsis charter. This charter is accepted by all members and defines clear participation rules. It states that the software kernel is free software and that all the source codes for all the models are shared by all the Capsis modellers, an efficient way to promote mutualization between modellers.

The Lusignan platform is dedicated to temporary grassland. It has been designed to increase our understanding of the effects of management of mixed arable crops / grasslands systems on the environmental outputs. The main scientific issue concerns the effects of grassland duration and management on SOM dynamics (quantity and composition), GHG emissions (CO2, N2O), nutrient lixiviation and functional biodiversity.