Each site has three common treatments: a control (C), a compacted treatment (T), a compacted and decompacted treatment (D), and more specific ones, locally decompacted soil (P) at Azerailles 54, and, limed (A) and limed and compacted (CA) at Clermont en Argonne 55. Each treatment plot of 0.25ha is replicated three times (3 blocks). Azerailles site was installed in spring 2007 and Clermont in spring 2008. Since that time monitoring occured for: -mesoclimate: rainfall, air temperature, relative humidity, bulk deposition (continuous recording) - soil climate: soil moisture (TDR Trase system (5 replicates at 15 and 60 cm depth) in one replicate of C and T (recorded each four hours) - water table: 2 piezometers in each replicate of treatments (Divers hourly recorded); monthly sampling using specific devices for Redox problems - weakly fixed soil water: cup lysimeters inserted at 15 and 60 cm depth (4 replicates in one block for C and T treatments) + teatments A and CA at Clermont en Argonne. Automate for constant vacuum at 700hP. Monthly collection for chemical analysis - soil gas: specific captors installed at 5, 10, 20, 35, 50 and 70 cm depth in each C and T treatments of the 3 blocks in the 2 sites. Monthly collection for chemical analysis (N, CO2, CH4, N2O, O2) -soil solid phase: soil morphology, soils physics, chemistry and biology (pro parte) were initialy described. Soil physical parameters (strengh, bulk density, hydraulic conductivity, swelling/shrinkage, porosity...), chemistry (C, N, exchangeable cations, P) and biology (earthworms) are perediodicaly investigated (each year for physics; 2/3 years for chemistry and biology). vegetation: both understorey and oak plantation were annually observed soil biology: earthworms are periodically observed

The Barbeau research facility is located in a 33-ha forest located nearby the Fontainebleau forest. Barbeau is a mature sessile Oak stand with a Hornbeam understory. Since 2005, a monitoring facility has continuously been measuring : - the exchanges of carbon and water vapour between the forest ecosystem and the atmosphere (through the eddy covariance (EC) methodology, installed at the top of a 35-m “flux” tower, and through organ-scale photosynthesis and respiration set-ups; 30-min time step) - the atmosphere and forest microclimates (above and within the canopy, including incoming, absorbed and reflected radiations in several spectral bands, temperatures, humidity; 30-min time step) - key variables for understanding forest functioning (e.g. tree diameter / biomass growth and soil water content on a hourly to weekly basis, tree organs nutrient contents on a seasonal basis, leaf area index on an annual basis etc.) The instruments are co-located in a 2500-m² fenced area. However, the monitored “footprints” of sole instruments ranges from a few cm3soil (e.g. measurements of soil temperature) up to hectares (e.g. integrated measurements of co2 and h2o exchanges with the EC method). Beside this ensemble of continuously monitored variables, spatial surveys of the stand characteristics (e.g. tree growth, soil properties and C/N contents, soil respiration, leaf area) and large-scale experiments (e.g. 13C-enriched CO2 labelling) are regularly conducted in Barbeau.

The Itatinga site is a long-term experimentation (started in 2008) that focus mainly on eucalyptus plantations. Our specific objectives are: - To understand the biogeochemical functioning of these fast-growing Eucalyptus plantations, and in particular the coupling between water, carbon, and nutrients cycles; - To use this information to develop eco-physiological models simulating the growth and balance of water, carbon and mineral elements of plantations; - To spatialize models from ground maps, satellite images and meteorological data; - To evaluate the environmental impacts and sustainability of plantations (impacts on water resources, soil fertility, regional climate. The experiment is located in Brazil, in the state of Sao Paulo. It is located at an average altitude of 800 m. Longitude / latitude 48°43'40.60''W / 22°58'4.50 ''S with an average annual temperature of 19.6 ° C and rainfall of the order of 1350 mm/year. The soil of the experiment is a ferralsol made of 72% sand, 15% clay, 3% silt. The site equipment includes a flux tower in a clonal Eucalyptus plantation, where nutrient cycles are also monitored. It also includes clonal tests, experimental plots that aim to study the effects of partial exclusion of rainfall and potassium fertilization on the cycles of mineral elements, carbon and water, and finally experimental plots comparing the ecophysiological / biogeochemical functioning of monospecific and plurispecific plantations. This study of the impact of tree diversity on the functioning of forest ecosystems is conducted on high-growing exotic species (mixture of Eucalyptus grandis and Acacia mangium) and on native species of Mata Atlântica (biome in which is located the Itatinga site). themes : Biogeochemistry of forest plantations, Ecological intensification of tropical forest plantations.

Aims and Philosophy of the CoffeeFlux Collaborative Platform The aim of Coffee-Flux is to assess carbon, nutrients, water and sediment Ecosystem Services (ES) at the scale of a coffee agroforestry watershed and additional experiments. Observation, experimentation, modelling and remote-sensing are combined, collecting data and calibrating models locally, then upscaling to larger regions. The project has been running continuously since 2009, in order to encompass seasonal and inter-annual fluctuations of coffee productivity and ecosystem services. Coffee-flux is a platform where collaborative research on coffee agroforestry is promoted: data are being shared between collaborators and positive interactions are enhanced. The philosophy is to concentrate several investigations on one specific site and for several years, to share a useful common experimental database, to develop modelling and to publish results in highly-ranked scientific journals. Applied research is also highly encouraged (e.g. C-Neutral certification, NAMA, Agronomy, etc.). Coffee-Flux benefits from infrastructure, easy access from CATIE and very good security, ready to welcome complementary scientific investigations and collaborations. The project is wide open to complementary projects, scientists and of course to students. The core data base is for sharing.

Rubberflux is a flux tower site aiming at providing a complete picture of CO2, water and energy budget of a rubber tree plantation using the eddy-covariance (EC) method combined with ground-based measurements of carbon/water stocks and flux among the different components of the ecosystem. This approach allows quantifying flux (NEE an ETR), partitioning them among tree organs, soil, understorey vegetation (etc.…), and eventually understanding the interactions with climate and the availability of soil resources (water, nutrients). This is a common approach to 3 other flux tower sites on tropical tree plantations managed by CIRAD’s researchers within the umbrella of the SOERE F-ORE-T network. The Rubberflux site is located in Thailand, about 140km east of Bangkok. It was set-up in 2006 in a 12 years rubber plantation of the Chachoengsao Rubber Research Centre (CRRC), a research facility of the Rubber Research Institute of Thailand (RRIT). The collaboration with CRRC staff has also permitted to implement studies on a chronosequence of rubber plantations. A CIRAD’s researcher has been permanently based in Thailand since then to ensure the functioning of the site in collaboration with the staff of CRRC and a research team from Kasetsart University (KU). The research works conducted on the Rubberflux site have been funded by CIRAD and KU with substantial contribution of the Thai Research Fund (TRF), the SOERE FORET and the French Embassy in Thailand.

The Estrées-Mons platform is dedicated to arable crops. It evaluates the effect of agricultural practices on C and N cycles in the soil-plant system and their interaction. Nitrate leaching, SOM evolution and GHG emissions (CO2, N2O) are monitored according to level of N intensification, crop residues export, soil tillage and legume frequency. The key issue is to understand how the wide variation in C and N inputs affects C and N cycles in more or less intensified systems.

The Lusignan platform is dedicated to temporary grassland. It has been designed to increase our understanding of the effects of management of mixed arable crops / grasslands systems on the environmental outputs. The main scientific issue concerns the effects of grassland duration and management on SOM dynamics (quantity and composition), GHG emissions (CO2, N2O), nutrient lixiviation and functional biodiversity.

Long term study of mixed forest of Pinus halepensis and Quercus ilex 55. Currently focused on carbon and water cycles, with routine measurements at the soil, plant, and ecosystem levels. Ecosystem manipulation consists in rainfall reduction and irrigation. Site is composed of : - a main enclosed area of 80x80 m ; - a 16 m tower supporting weather and eddy covariance measurements (ICOS level 2) - four 25x25 m plots (two inside and two outside the enclosed area) for 30% rainfall exclusion (with gutters), irrigation, control, and control with reversed gutters. In all plots, measurements are carried out for soil moisture, sap flow, stem diameter growth, leaf area index, litterfall, leaf water potential, and soil respiration.

The site of Montiers, localized at the boundaries between Meuse and Haute-Marne departments, North-East of France, has a large surface area (143 ha). It comprises two soil successions (toposequence) and the climate, stand conditions (age, species, forest management) are equivalent on all the surface of the site. The facility comprises three biogeochemical stations of 10 000 m2 each and one flux tower above forest canopy (45 m-high) settled along a soil succession representative of soils of the region. The three stations include four substations of which three are strongly equipped and one is free for future experimentations. Each equipped substation comprises lysimeters at different soil depths (litter, -10 cm, -30 cm, -60 cm and -90 cm; 3 replicates in general), tensio-lysimeters (-10 cm, -30 cm, -60 cm, -90 cm and -120 cm; 3 replicates in general) and temperature and moisture probes at different soil depths (-10 cm, -30 cm, -60 cm and -90 cm; 4 replicates), litterbags (6 replicates), stemflows (6 replicates), gutters (4 replicates). These stations allow to follow-up on the long term the flows of water, and major (Ca, Mg, K, Na, P, Fe, Mn, Si, Al, S, C, N) and trace (Cl, Se, B, I, Cs) elements between the different compartments (soil, tree, atmosphere) of a beech forest. Each station is settled on a different soil type, i.e., alocrisol, calci-brunisol, rendosol thus allowing to assess the impact of the soil type on biogeochemical cycles and on tree growth. The flux tower is equipped with a Eddy Covariance system (CO2, H2O, and sensible heat) and a complete set of sensors recording the aerial and edaphic meterological conditions, the phenology and the canopy status. The data from flux tower are available on demand. The forest mainly consists in a beech timber of about 50 years: dominant species and forestry in the region. The effect of the soil on the biogeochemical and biological functioning of this beech forest is dealt with a very integrated approach (ecophysiology, microbiology, soil science and biogeochemistry). In addition, the flux tower permits to measure, at various levels of the canopy and above, meteorological parameters (temperature, radiation, and precipitation), the gaseous exchanges and the particular deposits.