Metadata catalogue
Metagenomic analyses of the hydrothermal shrimp Rimicaris exoculata cephalothoracic epibiotic community
|
|
Citation proposal
Aube Johanne (IFREMER) - Cambon-Bonavita Marie-Anne (IFREMER) (2018) . Metagenomic analyses of the hydrothermal shrimp Rimicaris exoculata cephalothoracic epibiotic community. IFREMER https://services.mspdata.eu:/geonetwork/srv/api/records/4186eeef-32b1-4ffb-9a40-61a02a3852b7 |
Simple
- Date ( Creation )
- 2013-08-11
- Date ( Publication )
- 2018-03-22
- Identifier
- FR-330-715-368-00032-DATARMOR_BIOINFO-RIMICARIS
- Identifier
- DOI:10.12770/4186eeef-32b1-4ffb-9a40-61a02a3852b7
- Credit
- Ifremer - Laboratoire de Microbiologie des Environnements Extrêmes - Projets ABYSS, REMIMA and LabexMer
Author
Author
Publisher
- GEMET - INSPIRE themes, version 1.0 ( Theme )
-
- Habitats and biotopes
- Thèmes Sextant ( Theme )
-
- /Biological Environment/Bioinformatics
- Use limitation
- Public access
- Access constraints
- Copyright
- Use constraints
- Copyright
- Other constraints
- Spatial representation type
- Grid
- Metadata language
- English
- Character set
- UTF8
- Topic category
-
- Oceans
))
- Reference system identifier
- EPSG / WGS 84 (EPSG:4326) / 8.6
- Topology level
- Geometry only
- Number of dimensions
- 2
- Dimension name
- Column
- Dimension name
- Row
- Cell geometry
- Area
- Distribution format
-
- ( )
- OnLine resource
-
DOI of the dataset
DOI of the dataset
- OnLine resource
- /home/ref-bioinfo/ifremer/lmee/REX_Reveillaud
- OnLine resource
-
Download link
Download link
- Hierarchy level
- Dataset
- Statement
-
We collected Rimicaris exoculata specimens using a suction sampler manipulated by the Remote Operated Vehicle (ROV) Victor 6000 on board on the research vessel Pourquoi pas?. Samples were taken from three hydrothermal sites on the Mid Atlantic Ridge: Rainbow during the MoMAR and the Biobaz cruise, TAG and Snake Pit during the BICOSE cruise.
We performed DNA extraction of the six Rimicaris cephalothoracic appendages (2 branchiostegites + 2 scaphognathites + 2 exopodites).
We sheared DNA to 175 bp using a Covaris S-series sonicator and completed metagenomic library construction using the Ovation Ultralow Library DR multiplex system (Nugen) following manufacturer’s instructions. We performed metagenomic sequencing on an Illumina HiSeq 1000 and a NextSeq at the W.M. Keck sequencing facility at the Marine Biological Laboratory. All libraries were paired-end, with a 30 bp overlap, resulting in an average merged read length of 170 bp.
Metadata
- File identifier
- 4186eeef-32b1-4ffb-9a40-61a02a3852b7 XML
- Metadata language
- English
- Character set
- UTF8
- Hierarchy level
- Dataset
- Date stamp
- 2021-08-11T16:58:21
- Metadata standard name
- ISO 19115:2003/19139 - SEXTANT
- Metadata standard version
- 1.0
